The transfer vector was modified to allow for ligation-independent cloning (LIC) of PCR fragments. The baculovirus transfer vector pAcUW51 was altered to contain a honeybee melittin signal sequence after the polyhedrin promoter.
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Cloning, the process of generating a genetically identical copy of a cell or an organism. Cloning happens often in nature, as when a cell replicates itself asexually without genetic alteration or recombination. Learn more about cloning experiments, cloning techniques, and the ethics of human reproductive cloning.
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The pCR8/GW/TOPO vector enables efficient TOPO-TA cloning. These vectors easily facilitate multipurpose use: rapid recombination into a variety of Gateway Destination vectors, convenient sequencing, robust selection in E. coli with spectinomycin resistance, and easy exicision of insert DNA with flanking EcoR I sites (Figure 3). Figure 3.
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Vector Characteristics and Cloning Strategy In general, translation vectors are used for the expression of target genes derived from eukaryotic sources, and transcription vectors are used for target genes derived from prokaryotic genes (which usually carry compatible ribosome binding sites).
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Expression Systems The general aim is to express specific proteins in vitro in order to study genes and gene manipulation effects. The process starts with a gene of interest (GOI) and results in a plasmid that contains the necessary elements for propagating the plasmid/GOI in bacteria, and later expressing the GOI in either prokaryotic or eukaryotic cells.
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ADL Phage Display Vector Series. Vectors for easy cloning, adaptable display and versatile... Multiple tags and cloning sites. Varied display strategies.